A Comparison of Three Bioinformatics Pipelines for the Analysis of Preterm Gut Microbiota using 16S rRNA Gene Sequencing Data

نویسندگان

  • Erica Plummer
  • Jimmy Twin
  • Dieter M. Bulach
  • Suzanne M. Garland
  • Sepehr N Tabrizi
چکیده

1Murdoch Childrens Research Institute, The Royal Children’s Hospital, Flemington Rd, Parkville, Victoria 3052 Australia 2 Monash University, Victoria 3800, Australia 3The University of Melbourne, Victoria 3050, Australia 4Victorian Life Sciences Computation Initiative, The University of Melbourne, Parkville Campus, LAB-14, 700 Swanston St, Carlton, Victoria 3053, Australia 5The Royal Women’s Hospital, 20 Flemington Rd, Parkville, Victoria 3052, Australia 6The Royal Children’s Hospital, 50 Flemington Rd, Parkville, Victoria 3052, Australia

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Molecular identification and phylogenetic analysis of Lactobacillus and Bifidobacterium spp. isolated from gut of honeybees (Apis mellifera) from West Azerbaijan, Iran

Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) and phylogenetic analysis were used for molecular identification of lactic acid bacteria (LABs) isolated from Apis mellifera. Eighteen honeybee workers were collected from three different apiaries in West Azerbaijan. LABs from the gut of honeybees were isolated and cultured using routine biochemical proce...

متن کامل

Genetic variations of avian Pasteurella multocida as demonstrated by 16S-23S rRNA gene sequences comparison

Pasteurella multocida is known as an important heterogenic bacterial agent causes some severe diseases such as fowl cholera in poultry and haemorrhagic septicaemia in cattle and buffalo. A polymerase chain reaction (PCR) assay was developed using primers derived from conserved part of 16S-23S rRNA gene. The PCR amplified a fragment size of 0.7 kb using DNA from nine avian P. multocida  isolates...

متن کامل

Temporal probabilistic modeling of bacterial compositions derived from 16S rRNA sequencing

Motivation The number of microbial and metagenomic studies has increased drastically due to advancements in next-generation sequencing-based measurement techniques. Statistical analysis and the validity of conclusions drawn from (time series) 16S rRNA and other metagenomic sequencing data is hampered by the presence of significant amount of noise and missing data (sampling zeros). Accounting un...

متن کامل

Diversity of caprine and ovine Pasteurella multocida isolates based on 16S rRNA gene sequencing

In this study, to increase information about the relationship between caprine and ovine isolates of Pasteurella multocida, 16S rRNA gene sequencing of 9 goats (5) and sheep (4) isolates were investigated. Also, capsular type and toxAgene presentation was studied in this paper. All isolates, except one, belong to capsular type A, and toxA+ strain distributed among strains were isolated from both...

متن کامل

Reducing Viability Bias in Analysis of Gut Microbiota in Preterm Infants at Risk of NEC and Sepsis

Necrotising enterocolitis (NEC) and sepsis are serious diseases of preterm infants that can result in feeding intolerance, the need for bowel resection, impaired physiological and neurological development, and high mortality rates. Neonatal healthcare improvements have allowed greater survival rates in preterm infants leading to increased numbers at risk of developing NEC and sepsis. Gut bacter...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2016